Purification and some properties of progenitor toxins of Clostridium botulinum type B.

Cloning, Expression and Purification of Clostridium botulinum Neurotoxin Type E Binding Domain

Oral toxicities of Clostridium botulinum toxins in response to molecular size.

Clostridium botulinum type A, B, and F toxins of different molecular sizes were fed to mice to compare the oral toxicities. The progenitor toxin, a complex of a toxic and nontoxic component, of any type was higher in oral toxicity to mice than the dissociated toxic component or the derivative toxin. The former may no doubt play a more important role in the pathogenesis of food-borne botulism. T...

Characterization of Clostridium botulinum spores and its toxin in honey

Botulism is a serious paralytic disease caused by Clostridium botulinum toxin in foods. There are seven recognized serotypes of botulinum neurotoxins among which the principal prevalent types in humans include A, B and E. Infant botulism results from intestinal colonization and toxin production by C. botulinum spores in babies less than 1 year old. Honey is the most important food discriminated...

Studies on immunity to toxins of Clostridium botulinum. V. Detoxification of purified type A and type B toxins, and the antigenicity of univalent and bivalent aluminium phosphate adsorbed toxoids.

Fiock, Mary A. (Fort Detrick, Frederick, Md.), Allen Yarinsky, and James T. Duff. Studies on immunity to toxins of Clostridium botulinum. VII. Purification and detoxification of trypsin-activated type E toxin. J. Bacteriol. 82:66-71. 1961.-A procedure was described for production, purification, and conversion to toxoid of the Clostridium botulinum type E trypsin-treated toxin. A medium containi...

Expression and Purification of Neurotoxin-Associated Protein HA-33/A from Clostridium botulinum and Evaluation of Its Antigenicity

Background: Botulinum neurotoxin (BoNT) complexes consist of neurotoxin and neurotoxin-associated proteins. Hemagglutinin-33 (HA-33) is a member of BoNT type A (BoNT/A) complex. Considering the protective role of HA-33 in preservation of BoNT/A in gastrointestinal harsh conditions and also its adjuvant role, recombinant production of this protein is favorable. Thus in this study, HA-33 was expr...